Definition of SDN-1 and SDN-2 genome-edited plants, the difference between SDN1 and SDN2 genome-edited plants, and various
Difference between sdn1 and sdn2 genome edited plants
Point | SDN-1 | SDN-2 |
Main difference endogeneous or exogeneous | Involves the unguided repair of a targeted DNA break by the natural endogenous DNA repair mechanism of the host organism such as non-homologous end joining. | Involves a template-guided repair of a targeted DNA break using an externally supplied template sequence. |
Activity | The spontaneous repair of this break can lead to a mutation causing gene silencing, gene knock-out, or a change in the activity of a gene. | The donor carries one or several small mutations flanked by two sequences matching both ends of the DNA break and is thus recognized as a repair template, allowing the introduction of the mutation(s) at the target site. |
Edited plants obtained | The SDN-1 genome edited plants produced will be free from exogenous/foreign DNA. These mutations can be base substitutions/indels/deletions including large deletions or structural changes. | The resultant mutant carries a modified sequence, leading to an altered expression profile of the gene and/or altered activity of the encoded protein/RNA. |
Ultimate place in gene pool | These resultant mutations are comparable to those occurring in nature, obtained through conventional mutagenic treatments or natural variation found in the primary/secondary gene pool. | Thus, the edited version could be regarded as an allelic form comparable to those available in the primary/secondary gene pool |
FAQ on difference between SDN1 and SDN2 genome edited plants
SDN full form in genome editing crispr
SDN stands for Site-Directed Nuclease
which category of genome edited plants will be free from exogenous or foreign dna
The SDN-1 genome edited plants produced will be free from exogenous/foreign DNA
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